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Simultaneous Two-dimensional Separation Employing Planar Chromatography and Electrophoriesis

July 13, 2012

Writer: Bret E. Dunlap, Peter R. Stevenson, and David C. Collins, PhD

Planar chromatography and electrophoresis are one-dimensional separation techniques. In this work, planar chromatography and electrophoresis were carried out simultaneously in two separate dimensions. Chromatography occurred via capillary action while an orthogonal electric field was simultaneously applied to promote electrophoresis. Using copper wires, a custom-made planar chromatography plate with a novel dual solvent chamber was used to apply the electric field. Several sample types were analyzed; i.e., hydrophilic vitamins, amino acids, and dyes.

 

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