Presented by Paul Powell, Jared Breakall, Bryce Thompson, and David C. Collins

April 1, 2014
Writer: Brenda Pincock

Introduction

Since the era of "-omics", the need for more efficient and powerful separation of biological molecules in complex mixtures has
been on the rise. Repeatable two-dimensional separations of amino acids, vitamins, and dyes have been performed on thin
layer chromatography (TLC) plates using the novel technique of simultaneous chromatography and electrophoresis (SCE).
Past apparatus designs relied on modified TLC plates and capillary action to run separations (Figure 1); the new apparatus designs injects the mobile phase via syringe pump (Figure 2) which introduces a number of benefits and new complications.
The current work has been focused on optimizing the parameters affecting chromatographic separation. Methods of solvent delivery, pressure application, and electrode positioning have proven to be primary causes of inconsistency in the solvent flow. The current design simplifies all these problematic factors and has provided more consistent separations.
Since the era of "-omics", the need for more efficient and powerful separation of biological molecules in complex mixtures has been on the rise. Repeatable two-dimensional separations of amino acids, vitamins, and dyes have been performed on thin-layer chromatography (TLC) plates using the novel technique of simultaneous chromatography and electrophoresis (SCE).
Past apparatus designs relied on modified TLC plates and capillary action to perform separations (Figure 1). The newer designs inject mobile phase via syringe pump (Figures 2 - 8), providing benefits, and new complications.
Current work focuses on the optimization of parameters affecting chromatographic separation. Methods of solvent delivery, pressure application, and electrode positioning have been identified as primary causes of observed mobile phase flow  inconsistencies. These parameters have been modified significantly in the most recent design.

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