Simultaneous Chromatography and Electrophoresis: Apparatus Design and Development
Research Presentation by Paul Powell, Kei Furukawa, Jared Breakall, and David C. Collins
Writer: Brenda Pincock
Since the era of "-omics", the need for more efficient and powerful separation of biological molecules in complex mixtures has been on the rise. Repeatable two-dimensional separations of amino acids, vitamins, and dyes have been performed on thin layer chromatography (TLC) plates using the novel technique of simultaneous chromatography and electrophoresis (SCE). Past apparatus designs relied on modified TLC plates and capillary action to run separations (Figure 1); the new apparatus injects the mobile phase via syringe pump (Figures 2 and 3) which introduces a number of benefits and new complications. Current work has been focused on improving the chromatographic separation by generating a uniform solvent front. This work focuses on efforts to optimize parameters affecting flow, flow rate, seal, and applied pressure.