April 10, 2015
Writer: Brenda Pincock
Optimizing Flow Rate and Electric Potential in Pressurized Simultaneous Chromatography and Electrophoresis
Thin layer chromatography (TLC) and electrophoresis, with their long histories of simple and effective characterization of chemical mixtures, have motivated an effort to combine the techniques. Simultaneous chromatography and electrophoresis (SCE) utilizes an electric filed orthogonal to capillary action to achieve a single-step two-dimensional separation. In this work we investigate plate conditioning and introduce pressurized simultaneous chromatography and electrophoresis (pSCE). These improvements have reduced separation times and concurrently increased separation quality as described by Nearest Neighbor Distances (NND) analysis.