Examination of the pH Profile in Simultaneous Chromatography and Electrophoresis

Presented by Brae Petersen, Kishor Prasain, Tim Rose, and David C. Collins

December 13, 2013

Writer: Brenda Pincock

Simultaneous chromatography and electrophoresis (SCE) combines an electric field orthogonal to capillary action (Figure 1). Compounds are separated according to their charge-to-mass ratio and polarity. Electrolysis occurring at both electrodes creates acidic and basic regions. This work initiates an investigation into how these pH regions, in combination with the pH of the stationary and mobile phases, affect the chromatographic and electrophoretic separations.

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