Presented by Brae Petersen, Kishor Prasain, and David C. Collins Chemistry Department, Brigham Young University - Idaho

March 3, 2014
Writer: Brenda Pincock

Simultaneous chromatography and electrophoresis (SCE) combines an electric field orthogonal to capillary action (Figure 1). Compounds are separated according to their charge-to-mass ratio and polarity. Previous work has demonstrated that a buffered mobile phase is unable to alter the pH of the silica gel during analysis. Thus, separations are similar despite pH (not shown). This work investigates the effects of preconditioning TLC plates on SCE separations.

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