Evaluation of Separation Conditins for Simultaneous Electrophoresis and Chromatography
Writer: Peter R. Stevenson, Bret E. Dunlap, and David C. Collins, PhD
Planar chromatography and electrophoresis are one-dimensional separation techniques. In this work, various conditions were analyzed while employing simultaneous electrophoresis and chromatography (SEC) in two separate dimensions. SEC was previously characterized as a notable technique using vitamins, amino acids, and dyes . Chromatography occurs via capillary action while an orthogonal electric field is simultaneously applied to promote electrophoresis. A custom-made planar chromatography plate with a novel dual solvent chamber was used to apply the electric field.